DEVELOPMENT OF LIOPHYLIZED KIT OF TIN- GLUCOHEPTONATE FOR IN VITRO LABELING LEUCOCYTES WITH 99m-TECHNETIUM

Many radiopharmaceuticals are applied in the scintigraphic evaluation of inflammatory and infection. The inflammatory reaction represents the reaction of the organism to any traumatic condition and this process atracts leucocytes to the inflamatory focus (chemotaxy) and substances (enzymes, antibodies and complements) that represents the functions of defense and repair, respectively. Labeled leucocytes are recognized as the best radiopharmaceutical to the detection of inflammatory and infection. This work intended the development of a tin-glucoheptonate lyophilized kit to be applied in the in vitro labeling of leucocytes with 99m-technetium. The leucocytes labeling procedure using the kit was optimized and the principal parameters studied were the volume of the saline reconstituted tin-glucoheptonate kit, reaction incubation time and temperature in the pre-stanization procedure. Then the leucocytes were incubated with Tc (185 MBq), at different times at room temperature. Labeling yield were superior than 90%. Lyophilized tin-glucoheptonate kit was stable for 90 days. Scintigraphic images obtained at 1,2 e 3 hours after the administration of labeled human leucocytes in rabit with inflammatory focus promoted by tupertine injection showed high and persistent uptake of the labeled leucocytes in the lesion .